《植物生理学报》 2013, 49(4): 385-391

秋水仙素诱导细毡毛忍冬同源四倍体

陈泽雄^1,2, 吴林¹, 刘奕清^1.2,*

1重庆文理学院花卉研究所, 重庆402160; 2重庆市特色植物种苗工程技术研究中心, 重庆402160

通信作者：刘奕清；E-mail: liung906@163.com；Tel: 023-49685199

摘　要：

以细毡毛忍冬叶片为外植体建立了离体再生体系, 在此基础上, 以叶片诱导出愈伤组织, 用秋水仙素诱导其同源四倍体。结果表明, 秋水仙素浓度为0.2 g·L-1, 液体培养48 h, 四倍体诱导率最高为15.0%。四倍体细毡毛忍冬生长缓慢, 植株节间短, 茎干粗壮, 叶片颜色较二倍体浓绿, 叶形指数减少, 保卫细胞增大, 气孔密度减少, 绿原酸含量增加。

关键词：细毡毛忍冬; 秋水仙素; 同源四倍体; 倍性鉴定

收稿：2013-01-04   修定：2013-03-20

资助：重庆市高校优秀成果转化项目(KJZH11220)、重庆文理学院校级科研项目(Y2010YL40)和重庆文理学院特色林木 种质资源创新重点实验室平台建设项目。

Autotetraploid Induction in Lonicera similis by Colchicine

CHEN Ze-Xiong^1,2, WU Lin¹, LIU Yi-Qing^1,2,*

¹Flower Research Institute, Chongqing University of Arts and Sciences, Chongqing 402160, China; ²Engineering Research Center for Special Plant Seedlings, Chongqing 402160, China

Corresponding author: LIU Yi-Qing; E-mail: liung906@163.com; Tel: 023-49685199

Abstract:

To establish an effective leaf generation system and induce stable tetraploid of Lonicera similis, cal-luses were induced by using leaves of L. similis as explants and calluses were employed to establish the techni-cal system of autotetraploid induction in L. similis by colchicines. The results showed that the highest induction rate of tetraploid, 15.0%, was obtained from the callus cultured in the liquid medium supplemented with 0.2 g·L^-1 colchicine for 48 h. The plants grew slowly and showed the general characteristics of tetraploids, such as shorter internodal, thicker stem, deeper leaf, smaller leaf index, larger guard cells and less stomata density. The contents of chlorogenic acid of tetraploid plants were higher than diploid plants.

Key words: Lonicera similis Hemsl.; colchicines; autotetraploid; chromosome identification